小型化无透镜微流控片上生物成像检测

陈津; 黄汐威; 李阳波; 刘季璇; 孙玲玲

doi:10.11959/j.issn.2096-3750.2019.00111

您当前的位置：

首页 >

文章列表页 >

小型化无透镜微流控片上生物成像检测

理论与技术 | 更新时间：2024-06-05

- 小型化无透镜微流控片上生物成像检测
- Miniaturized lensless microfluidic on-chip bio-imaging detection
- 物联网学报 2019年3卷第2期页码：9-19
- 作者机构：
- 作者简介：
  
  [ "陈津（1992- ），男，福建三明人，杭州电子科技大学硕士生，主要研究方向为微流控片上成像电路与系统设计和声表面波传感检测。" ]
  [ "黄汐威（1987- ），男，江西上饶人，博士，杭州电子科技大学副教授，主要研究方向为CMOS集成传感器和微流控分析系统。" ]
  [ "李阳波（1994- ），男，山西朔州人，杭州电子科技大学硕士生，主要研究方向为CMOS图像传感检测和无透镜全息成像。" ]
  [ "刘季璇（1996- ），女，黑龙江齐齐哈尔人，杭州电子科技大学硕士生，主要研究方向为基于深度学习的片上细胞成像智能处理。" ]
  [ "孙玲玲（1956- ），女，山东泗水人，杭州电子科技大学射频电路与系统教育部重点实验室主任、教授，主要研究方向为微波毫米波集成电路与智能系统设计。" ]
- 基金信息：
  
  国家自然科学基金资助项目;The National Natural Science Foundation of China(61827806);国家自然科学基金资助项目;The National Natural Science Foundation of China(61501156);浙江省钱江人才计划D类项目;Qianjiang Talent Project Type-D of Zhejiang(QJD1802021);浙江省公益性技术应用研究计划基金资助项目;Public Welfare Technology Application Research Plan of Zhejiang Province(2017C31064)
- DOI：10.11959/j.issn.2096-3750.2019.00111
  中图分类号： TP391
- 纸质出版日期：2019-06-30，
  
  网络出版日期：2019-06，
- 稿件说明：
移动端阅览
陈津, 黄汐威, 李阳波, 等. 小型化无透镜微流控片上生物成像检测[J]. 物联网学报, 2019,3(2):9-19.

JIN CHEN, XIWEI HUANG, YANGBO LI, et al. Miniaturized lensless microfluidic on-chip bio-imaging detection. [J]. Chinese journal on internet of things, 2019, 3(2): 9-19.
陈津, 黄汐威, 李阳波, 等. 小型化无透镜微流控片上生物成像检测[J]. 物联网学报, 2019,3(2):9-19. DOI： 10.11959/j.issn.2096-3750.2019.00111.

JIN CHEN, XIWEI HUANG, YANGBO LI, et al. Miniaturized lensless microfluidic on-chip bio-imaging detection. [J]. Chinese journal on internet of things, 2019, 3(2): 9-19. DOI： 10.11959/j.issn.2096-3750.2019.00111.

摘要

用于生物医疗成像的传统高分辨率光学显微镜由于复杂的光学透镜系统导致高成本、不便携。为了实现小型化成像检测，基于CMOS图像传感芯片与微流控集成的无透镜微流控片上成像系统近年来发展迅速，是解决小型化显微成像检测的重要方法。综述了无透镜微流控片上成像系统的结构、成像原理、存在的问题与改进的方法，主要包括阴影成像系统、全息成像系统、荧光成像系统以及彩色成像系统等，分析了无透镜微流控片上成像系统的不足和可能的发展方向。

Abstract

The conventional high-resolution optical microscopy for biomedical imaging has high cost and low portability due to bulky optical lenses system.To realize miniaturized imaging

the recent advance of lensless microfluidic on-chip imaging technique based on the integration of CMOS image sensor and microfluidics has provided one promising solution.Therefore

the architecture

principle

problems and improvement solutions for different miniaturized lensless microfluidic on-chip bio-imaging

including shadow imaging

holographic imaging

fluorescent imaging and color imaging were reviewed.And the deficiencies and possible development directions were discussed.

关键词

片上成像无透镜微流控CMOS图像传感器小型化

Keywords

on-chip imaginglenslessmicrofluidicCMOS image sensorminiaturization

references

GABOR D . A new microscopic principle[J]. Nature, 1948,161(4098):777.

MUDANYALI O, OZTOPRAK C, TSENG D ,et al. Detection of waterborne parasites using field-portable and cost-effective lensfree microscopy[J]. Lab on a Chip, 2010,10(18): 2419-2423.

WU Y C, SHILEDAR A, LI Y C ,et al. Air quality monitoring using mobile microscopy and machine learning[J]. Light-Science ＆ Applications, 2017,6:12.

BALAKRISHNAN P, DUNNE M, KUMARASAMY N ,et al. An inexpensive,simple,and manual method of CD4 T-cell quantitation in HIV-infected individuals for use in developing countries[J]. JAIDS—Journal of Acquired Immune Deficiency Syndromes, 2004,36(5): 1006-1010.

DITTRICH P S, MANZ A . Lab-on-a-chip:microfluidics in drug discovery[J]. Nature Reviews Drug Discovery, 2006,5(3): 210-218.

LEE S, ONCESCU V, MANCUSO M ,et al. A smartphone platform for the quantification of vitamin D levels[J]. Lab on a Chip, 2014,14(8): 1437-1442.

STARKUVIENE V, PEPPERKOK R . The potential of high-content high-throughput microscopy in drug discovery[J]. British Journal of Pharmacology, 2007,152(1): 62-71.

GREENBAUM A, LUO W, SU T W ,et al. Imaging without lenses:achievements and remaining challenges of wide-field on-chip microscopy[J]. Nature Methods, 2012,9(9): 889-895.

GOROCS Z, OZCAN A . On-chip biomedical imaging[J]. IEEE Reviews in Biomedical Engineering, 2013,6: 29-46.

ISIKMAN S O, BISHARA W, MUDANYALI O ,et al. Lensfree on-chip microscopy and tomography for biomedical applications[J]. IEEE Journal of Selected Topics in Quantum Electronics, 2012,18(3): 1059-1072.

YUAN X, PU Y C . Parallel lensless compressive imaging via deep convolutional neural networks[J]. Optics Express, 2018,26(2): 1962-1977.

LUO W, GREENBAUM A, ZHANG Y B ,et al. Synthetic aperture-based on-chip microscopy[J]. Light-Science ＆ Applications, 2015,4:9.

ALEXANDROV S A, HILLMAN T R, GUTZLER T ,et al. Synthetic aperture fourier holographic optical microscopy[J]. Physical Review Letters, 2006,97(16):4.

WEI Q S, MCLEOD E, QI H F ,et al. Lensfree holographic cytometry using plasmonic nanoparticles[C]// 2013 IEEE Photonics Conference. IEEE, 2013: 3-4.

HUANG X W, GUO J H, WANG X L ,et al. A contact-imaging based microfluidic cytometer with machine-learning for single-frame super-resolution processing[J]. Plos One, 2014,9(8):10.

DELACROIX R, MOREL S N A, HERVE L ,et al. Cerebrospinal fluid lens-free microscopy:a new tool for the laboratory diagnosis of meningitis[J]. Scientific Reports, 2017,7:8.

OZCAN A, MCLEOD E . Lensless imaging and sensing[J]. Annual Review of Biomedical Engineering, 2016,18: 77-102.

SU T W, SEO S, ERLINGER A ,et al. High-throughput lensfree imaging and characterization of a heterogeneous cell solution on a chip[J]. Biotechnology and Bioengineering, 2009,102(3): 856-868.

SU T W, SEO S, EDINGER A ,et al. High-throughput cellimaging,counting and characterization on a chip[M]. New York: Amer Soc Mechanical EngineersPress, 2009.

HUANG X, FAROOQ U, CHEN J ,et al. A surface acoustic wave pumped lensless microfluidic imaging system for flowing cell detection and counting[J]. IEEE Transactions on Biomedical Circuits and Systems, 2017,11(6): 1478-1487.

黄汐威, 程涛, 丁诚翔 ,等. 无透镜微流控成像流动细胞检测与计数系统[J]. 传感器与微系统, 2017,36(5): 94-98.

HUANG X W, CHENG T, DING C X ,et al. Lensless microfluidic imaging flow cell detection and counting system[J]. Transducer and Microsystem Technologies, 2017,36(5): 94-98.

ZHENG G A, LEE S A, ANTEBI Y ,et al. The ePetri dish,an on-chip cell imaging platform based on subpixel perspective sweeping microscopy(SPSM)[J]. Proceedings of the National Academy of Sciences of the United States of America, 2011,108(41): 16889-16894.

HENG X, ERICKSON D, BAUGH L R ,et al. Optofluidic microscopy—a method for implementing a high resolution optical microscope on a chip[J]. Lab on a Chip, 2006,6(10): 1274-1276.

LEE L M, CUI X Q, YANG C H . The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts[J]. Biomedical Microdevices, 2009,11(5): 951-958.

ROY M, SEO D, OH S ,et al. A review of recent progress in lens-free imaging and sensing[J]. Biosensors ＆ Bioelectronics, 2017,88: 130-143.

BISHARA W, SIKORA U, MUDANYALI O ,et al. Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array[J]. Lab on a Chip, 2011,11(7): 1276-1279.

CHO C, CHOI B, KANG H ,et al. Numerical twin image suppression by nonlinear segmentation mask in digital holography[J]. Optics Express, 2012,20(20): 22454-22464.

SEO D, OH S, LEE M ,et al. A field-portable cell analyzer without a microscope and reagents[J]. Sensors, 2018,18(1):14.

XU W, JERICHO M H, MEINERTZHAGEN I A ,et al. Digital in-line holography for biological applications[J]. Proceedings of the National Academy of Sciences of the United States of America, 2001,98(20): 11301-11305.

XU W, JERICHO M H, MEINERTZHAGEN I A ,et al. Digital in-line holography of microspheres[J]. Applied Optics, 2002,41(25): 5367-5375.

GARCIA-SUCERQUIA J, XU W B, JERICHO S K ,et al. Digital in-line holographic microscopy[J]. Applied Optics, 2006,45(5): 836-850.

NOOM D W E, EIKEMA K S E, WITTE S . Lensless phase contrast microscopy based on multiwavelength Fresnel diffraction[J]. Optics Letters, 2014,39(2): 193-196.

BANYASZ I, KORNIS J . High-resolution lensless Fourier-transform digital holography[J]. International Society for Optical Engineering, 2005,5856: 71-79.

FIENUP J R . Phase retrieval algorithms:a comparison[J]. Applied Optics, 1982,21(15): 2758-2769.

FAULKNER H M L, RODENBURG J M . Movable aperture lensless transmission microscopy:a novel phase retrieval algorithm[J]. Physical Review Letters, 2004,93(2):4.

GARCIA-SUCERQUIA J, XU W B, JERICHO M H ,et al. Immersion digital in-line holographic microscopy[J]. Optics Letters, 2006,31(9): 1211-1213.

SEO S, SU T W, TSENG D K ,et al. Lensfree holographic imaging for on-chip cytometry and diagnostics[J]. Lab on a Chip, 2009,9(6): 777-787.

GARCIA-SUCERQUIA J . Color digital lensless holographic microscopy:laser versus LED illumination[J]. Applied Optics, 2016,55(24): 6649-6655.

LUO W, GREENBAUM A, ZHANG Y B ,et al. High-resolution on-chip imaging using synthetic aperture[C]// 2015 Conference on Lasers and Electro-Optics. IEEE, 2015.

ISIKMAN S O, BISHARA W, SIKORA U ,et al. Field-portable lensfree tomographic microscope[J]. Lab on a Chip, 2011,11(13): 2222-2230.

GREENBAUM A, SIKORA U, OZCAN A . Field-portable wide-field microscopy of dense samples using multi-height pixel super-resolution based lensfree imaging[J]. Lab on a Chip, 2012,12(7): 1242-1245.

GRANERO L, FERREIRA C, ZALEVSKY Z ,et al. Single-exposure super-resolved interferometric microscopy by RGB multiplexing in lensless configuration[J]. Optics and Lasers in Engineering, 2016,82: 104-112.

FEIZI A, ZHANG Y B, GREENBAUM A ,et al. Yeast viability and concentration analysis using lens-free computational microscopy and machine learning[C]// Optics and Biophotonics in Low-Resource Settings III Spie-Int Soc Optical Engineering, 2017.

DALOGLU M U, OZCAN A . Computational imaging of sperm locomotion[J]. Biology of Reproduction, 2017,97(2): 182-188.

DI CAPRIO G, GIOFFRE M A, SAFFIOTI N ,et al. Quantitative label-free animal sperm imaging by means of digital holographic microscopy[J]. IEEE Journal of Selected Topics in Quantum Electronics, 2010,16(4): 833-840.

SOLER C, PICAZO-BUENO J A, MICO V ,et al. Effect of counting chamber depth on the accuracy of lensless microscopy for the assessment of boar sperm motility[J]. Reproduction,Fertility,and Development, 2018,30(6): 924-934.

LI G X, ZHANG R B, YANG N ,et al. An approach for cell viability online detection based on the characteristics of lensfree cell diffraction fingerprint[J]. Biosensors ＆ Bioelectronics, 2018,107: 163-169.

SANZ M, PICAZO-BUENO J A, GARCIA J ,et al. Dual-mode holographic microscopy imaging platform[J]. Lab on a Chip, 2018,18(7): 1105-1112.

AXELROD D . Total internal reflection fluorescence microscopy in cell biology[J]. Traffic (Copenhagen,Denmark), 2001,2(11): 764-774.

COSKUN A F, SENCAN I, SU T W ,et al. Lensfree fluorescent on-chip imaging of transgenic caenorhabditis elegans over an ultra-wide field-of-view[J]. Plos One, 2011,6(1):9.

GREENBAUM A, ZHANG Y B, FEIZI A ,et al. Wide-field computational imaging of pathology slides using lens-free on-chip microscopy[J]. Science Translational Medicine, 2014,6(267):10.

SASAGAWA K, KIM S H, MIYAZAWA K ,et al. Dual-mode lensless imaging device for digital enzyme linked immunosorbent assay[C]// Frontiers in Biological Detection:From Nanosensors to Systems Vi Spie-Int Soc Optical Engineering, 2014.

IMAI K, NISHIGAKI M, ONOZUKA Y ,et al. A lens-freesingle-shot fluorescent imaging system using CMOS image sensors with dielectric multilayer filter[M]. Piscataway: IEEE PressPress, 2017.

LEE S A, OU X Z, LEE J E ,et al. Chip-scale fluorescence microscope based on a silo-filter complementary metal-oxide semiconductor image sensor[J]. Optics Letters, 2013,38(11): 1817-1819.

MUDRABOYINA A K, BLOCKSTEIN L, LUK C C ,et al. A novel lensless miniature contact imaging system for monitoring calcium changes in live neurons[J]. IEEE Photonics Journal, 2014,6(1):15.

BIGGS D S, ANDREWS M . Acceleration of iterative image restoration algorithms[J]. Applied Optics, 1997,36(8): 1766-1775.

COSKUN A F, SU T W, OZCAN A . Wide field-of-view lens-free fluorescent imaging on a chip[J]. Lab on a Chip, 2010,10(7): 824-827.

HAN C, PANG S, BOWER D V ,et al. Wide field-of-view on-chip talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator[J]. Analytical Chemistry, 2013,85(4): 2356-2360.

TAKEHARA H, NAGASAKI M, SASAGAWA K ,et al. Micro-light-pipe array with an excitation attenuation filter for lensless digital enzyme-linked immunosorbent assay[J]. Japanese Journal of Applied Physics, 2016,55(3):9.

AMOLINS K, ZHANG Y, DARE P . Wavelet based image fusion techniques-an introduction,review and comparison[J]. ISPRS Journal of Photogrammetry and Remote Sensing, 2007,62(4): 249-263.

LEE S A, ERATH J, ZHENG G A ,et al. Imaging and identification of waterborne parasites using a chip-scale microscope[J]. Plos One, 2014,9(2):5.

PANG S, CUI X Q, DEMODENA J ,et al. Implementation of a color-capable optofluidic microscope on a RGB CMOS color sensor chip substrate[J]. Lab on a Chip, 2010,10(4): 411-414.

GREENBAUM A, FEIZI A, AKBARI N ,et al. Wide-field computational color imaging using pixel super-resolved on-chip microscopy[J]. Optics Express, 2013,21(10): 12469-12483.

GREENBAUM A, AKBARI N, FEIZI A ,et al. Field-portable pixel super-resolution colour microscope[J]. Plos One, 2013,8(9):9.

WU Y C, ZHANG Y B, LUO W ,et al. Demosaiced pixel super-resolution for multiplexed holographic color imaging[J]. Scientific Reports, 2016,6:9.

ZHANG Y B, WU Y C, ZHANG Y ,et al. Fusion of lens-free microscopy and mobile-phone microscopy images for high-color-accuracy and high-resolution pathology imaging[C]// Optics and Biophotonics in Low-Resource Settings III Spie-Int Soc Optical Engineering, 2017.

MCLEOD E, NGUYEN C, HUANG P ,et al. Tunable vapor-condensed nanolenses[J]. ACS Nano, 2014,8(7): 7340-7349.

FEIZI A, ZHANG Y B, GREENBAUM A ,et al. Rapid,portable and cost-effective yeast cell viability and concentration analysis using lensfree on-chip microscopy and machine learning[J]. Lab on a Chip, 2016,16(22): 4350-4358.

HUANG X W, WANG X L, YAN M ,et al. A robust recognition error recovery for micro-flow cytometer by machine-learning enhanced single-frame super-resolution processing[J]. Integration—The VLSI Journal, 2015,51: 208-218.

COSTA F F . Big data in biomedicine[J]. Drug Discovery Today, 2014,19(4): 433-440.

浏览量

407

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于方形螺旋谐振器的紧凑型RFID标签天线